Association between depression and anxiety symptoms with physical activity among rural returning adolescents / 中国学校卫生

Objective@#To understand the symptoms of depression and anxiety among rural returning adolescents and to analyze their association with physical activity related factors, so as to provide reference for interventions targeting depression and anxiety symtoms in the population.@*Methods@#From April to June 2020, 3 495 middle school students were selected from 6 counties and districts of Shangrao City by random cluster stratified sampling sampling. The Patient Health Questionnaire-9 (PHQ-9) and Generalized Anxiety Disorder-7 (GAD-7) were used to evaluate the depressive and anxiety symptoms among middle school students. The International Physical Activity Questionnaire was used to assess their physical activity levels during the past week. Chi square test and Logistic regression analysis were used to determine the strength of the association between depression and anxiety symptoms and physical activity related factors in returning and non returning adolescents as well as the overall population.@*Results@#Univariate analysis showed significant differences in the prevalence of depressive and anxiety symptoms between rural returning and non returning adolescents and the overall population in terms of "type of school" "family economic situation" "parental occupation" "number of sports classes per week" and "level of physical activity per week" ( χ 2=78.21, 16.56, 135.44, 107.75, 7.10, 8.62; 97.94, 24.26 , 124.07, 90.36, 9.60, 8.34, P <0.05). Multivariate regression analysis showed a correlation between the occurrence of depression and the number of sports classes per week for rural returning and non returning adolescents and the overall population (number of sports classes per week for non returning was 2 times, OR=1.22, 95%CI =1.01-1.49; returning adolescents for 1 time, OR=1.85, 95%CI =1.06-3.23; the overall population for 1 time, OR=1.34, 95%CI =1.01-1.77 and 2 times, OR=1.20, 95%CI =1.01-1.43, P <0.05). There was a correlation between anxiety symptoms and the number of sports classes per week for returning adolescents (number of sports classes per week for returning adolescents was 1 time, OR=2.10, 95%CI=1.21-3.63, P < 0.05 ).@*Conclusion@#A low frequency of weekly sports classes may be a risk factor for depressive or anxiety symptoms in rural and returning adolescents. Rural primary and secondary schools should appropriately increase the number of physical education courses or arrange sports extended classes to promote the development of adolescent mental health.

Studies on phenylpropanoids from Eleocharis dulcis and their hepatoprotective activities / 中国中药杂志

To study phenylpropanoids from Eleocharis dulcis and their hepatoprotective activities. The compounds were separated and purified from ethyl acetate part by conventional column chromatography and preparative liquid chromatography, and their structures were identified by various spectral techniques. The HL-7702 cells damage model of hepatocytes induced by APAP was used to screen and evaluate the hepatoprotective activities of these compounds. Sixteen compounds were isolated from ethyl acetate part of E. dulcis, and their structures were identified as 6'-(4″-hydroxy-3″-methoxy-phenylpropenyl)-1-(10-methoxy-phenylacetone)-1'-O-β-D-glucopy-ranoside(1), susaroyside A(2), clausenaglycoside B(3), clausenaglycoside C(4), clausenaglycoside D(5), emarginone A(6), emarginone B(7), thoreliin B(8), 4-O-(1',3'-dihydroxypropan-2'-yl)-dihydroconiferyl alcohol 9-O-β-D-glucopyranoside(9), 2-[4-(3-methoxy-1-propenyl)-2-methoxy-phenoxy]-propane-1,3-diol(10), 6'-O-(E-cinnamoyl)-coniferin(11), methyl 3-(2-O-β-D-glucopyranosyl-3,4,5,6-tetramethoxyphenyl) propanoate(12), clausenaglycoside A(13), 9-O-(E-cinnamoyl)-coniferin(14), 6'-O-(E-cinnamoyl)-syringin(15), 2'-O-(E-cinnamoyl)-syringin(16). Among them, compound 1 was a new compound. Compounds 2-16 were isolated from this plant for the first time. Among them, compounds 2 and 8 showed certain hepatoprotective activities.

Analysis of Banxia Xiexintang Based on Ancient Literatures / 中国实验方剂学杂志

Banxia Xiexin Tang is one of the classic prescriptions for treating digestive system diseases.In this paper，using the method of bibliometrics，information of Banxia Xiexintang in ancient Chinese medical literatures were collected and screened out 399 effective data from 238 kinds of ancient books．Based on the statistics and analysis of the history，drug composition，main disease and syndrome，principle of prescription，dosage，processing，preparation，decocting and taking methods of Banxia Xiexin Tang，it is found that Banxia Xiexin Tang originated from Treatise on Febrile and Miscellaneous Disease written by ZHANG Zhong-jing，a famous physician in the Eastern Han dynasty，it is composed of seven herbs，namely，Pinelliae Rhizoma，Scutellariae Radix，Zingiberis Rhizoma，Ginseng Radix et Rhizoma，Glycyrrhizae Radix et Rhizoma Praeparata cum Melle，Coptidis Rhizoma，and Jujubae Fructus. It was mainly used to treat pizheng of mixed cold and heat. Most of the Banxia Xiexin Tang recorded in later generations follow the prescription composition and indications in Treatise on Febrile and Miscellaneous Disease and its clinical application has been extended and expanded，among the 352 literatures with the main diseases and syndromes recorded，the most common （341） were pizheng，accounting for about 96.88%，in addition，it is also used sporadically for shuzheng，malaria，nausea，damp-wen，jaundice，etc. Among the 122 documents with drug dosage records，nearly half of them are identical with the original records in Treatise on Febrile and Miscellaneous Disease. Among the 112 literatures with pharmaceutical processing，licorice （86） was the most，most of them were roasted （80），followed by pinellia ternata （79），and most of them were "decoction washing and sliding" （67）. Among the 111 documents recorded in the decoction method，most of them inherited Treatise on Febrile and Miscellaneous Disease by "removing the dross and again cooking" （68），there are also "Water Decoction" （32） and "Ginseng Radix et Rhizoma and Jujubae Fructus Decoction" （11）. Among the 108 documents with the method of taking medicine，nearly half of them inherited Treatise on Febrile and Miscellaneous Disease，which is "a liter of warm taking ，three times daily". Based on the ancient Chinese medical literatures，Banxia Xiexintang was systematically analyzed in order to provide more accurate ancient literature reference for the clinical application and development of classic prescriptions.

The design and implementation of power control circuit of ultrasonic scalpel based on DSP / 中国医学装备

Objective:To independently develop a power control system of ultrasonic scalpel so as to reduce the energy consumption and maintain the normal temperature of ultrasonic scalpel.Methods:In this paper, the model of equivalent circuit of ultrasonic transducer nearby syntony was built up, and the hardware control system of ultrasonic scalpel based on digital signal processing (DSP) was designed.Results:Through testing the circuit and work performance of power control system, the series of parameters such as effective value and so on which were produced by this system could adjust frequency of power source in time and attain anticipative functional indicator, and it took the ultrasonic scalpel to work in syntonic situation.Conclusion:The tested indicators of power control system of ultrasonic scalpel based on the kernel design of DSP can attain anticipative requirement, and can take this system to work in syntonic situation.

rhGM-CSF subcutaneously combined with rhIL-2 intravenous drip for relieving radiation esophagitis in patients with esophageal cancer undergoing radiotherapy / 肿瘤

Objective: To investigate the effect of subcutaneous injection of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) combined with intravenous drip of recombinant human interleukin-2 (rhIL-2) on radiation esophagitis in the patients with esophageal cancer undergoing radiotherapy. Methods: Seventy patients with esophageal cancer were randomly divided into the experimental group and the control group. Thirty-five patients in the experimental group were treated with subcutaneous injection of rhGM-CSF combined with intravenous drip of rhIL-2 and radiotherapy, while 35 patients in the control group were just treated with radiotherapy alone. The occurrence of radiation esophagitis, the degree of pain, intervention measures and treatment compliance were compared between the two groups. Results: There were 3 cases with grade 3 or 4 radiation esophagitis in the experimental group, and 12 cases in the control group (P < 0.05). The number of patients with swallowing pain (more than 7 points) caused by radiation esophagitis in the experimental group was significantly less than that in the control group (P < 0.05). The number of patients used the intervention drugs of radiation esophagitis in the experimental group was significantly less than that in the control group (P < 0.05). After the treatment, the nausea and vomiting, loss of appetite, pain, and insomnia in the experimental group were significantly better than those in the control group (all P < 0.05). There was no interruption of radiotherapy in the experimental group, while the radiotherapy of 3 cases in the control group was interrupted (P < 0.05). Conclusion: Subcutaneous injection of rhGM-CSF combined with intravenous drip of rhIL-2 significantly reduce the proportion of radiation esophagitis-related symptoms in the patients with esophageal cancer, improve the quality of life by reliving the swallowing pain, as well as improve the treatment compliance of patients.

Effects of obesity on global genome DNA methylation and gene imprinting in mouse spermatozoa / 中华男科学杂志

Objective@#To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA.@*METHODS@#We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO.@*RESULTS@#No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93.73 vs 97.26%, P = 0.252), H19 (98.00 vs 97.83%, P = 0.920), IGF2 (97.34 vs 96.25%, P =0.166), IGF2R (1.43 vs 1.11%, P = 0.695), PEG3 (0.19 vs 0.38%, P = 0.537), MEST (0.23 vs 0.68%, P = 0.315), NNAT (0.31 vs 0.00%, P = 0.134), or SNRPN (1.88 vs 3.13%, P = 0.628). A total of 8 942 DMRs were detected across the sperm genome (P <0.05). Gene functional enrichment analysis indicated that the enriched terms with the largest numbers of genes were the metabolic process (n = 1 482), RNA synthesis (n = 779), and transcription (n = 767).@*CONCLUSIONS@#The methylation level underwent no significant change in the DMRs of the imprinted genes from the mice with HFDIO, but the CG methylation of the genes involved in the metabolic process, RNA synthesis and transcription were significantly altered.

Studies on flavonoids from Cirsium setosum / 中国中药杂志

To investigate the chemical constituents of ethyl acetate from Cirsium setosum, fifteen flavonoids were obtained by column chromatography on silica gel, MCI, Sephadex LH-20, and preparative HPLC. Their structures were identified as 4',5,6-trihydroxy-7-methoxyflavone(1), 4',5-dihydroxy-7,8-dimethoxyflavone(2), sorbifolin-6-O-β-glucopyranoside(3), kaempferol-7-O-α-L-rhamnoside(4), kaempferol(5), quercetin-3-O-β-D-glucosyl-7-O-α-L-rhamnoside(6), myricetin(7), myricetin-3-O-β-D-glucoside(8), 5,7- dihydroxy -3',4'- dimethoxyflavone(9), 3',4',5- trihydroxy-3,7-dimethoxyflavone(10), 3',3,4',5-tetrahydroxy-7-methoxyflavone(11), 3'-hydroxy-4',5,7-trimethoxyflavone(12), 7-hydroxy-3',4',5-trimethoxyflavone(13), 4',5-dihydroxy-2',3',7,8-tetramethoxylflavone(14), and 5-hydroxy-2',3',7,8-tetramethoxylflavone(15) by spectroscopic data analysis. All compounds were isolated from this plant for the first time.Compounds(1-15) were evaluated for their hypoglycemic activities by PTP1B enzyme model. Among them, compounds 2, 12, and 14 showed significant PTP1B inhibitory activities with IC₅₀ values of 2.54, 1.85, 2.11 μmol•L⁻¹, respectively.

The correlation of age with the risk of multiple organ dysfunction syndrome in the elderly patients with congestive heart failure and hypertension / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To study the prediction value of age and congestive heart failure (CHF) for occurrence of multiple organ dysfunction syndrome elderly(MODSE) in old patients with hypertension.</p><p><b>METHODS</b>Medical history of 19,996 cases (aged over 60 year) admitted to PLA General Hospital because of hypertension or developing hypertension during hospital stay from Jan 1993 to Dec 2008 were analyzed retrospectively. According to age the patients were divided into four groups: 60-69 year group; 70-79 year group; 80-89 year group; > or = 90 year older group. The incidence of CHF and the morbidity of MODSE induced by CHF at different ages and different boundary ages were investigated.</p><p><b>RESULTS</b>1. The incidence of MODSE in CHF cases was higher than that in the non-CHF cases (7.43% versus 3.05%, Chi(2) 195.15, P < 0.01), showing CHF were the important factor in happening of MODSE. 2. The incidence of CHF and the morbidity of MODSE were 10.60% versus 18.88% versus 30.11% versus 60.57%, P <0.05, P < 0.05 and 1.6 versus 7.0 versus 17.08 versus 25.47% , in 60-69 year group; 70-79 year group; 80-89 year group; > or =90 year older group, P < 0.05. Occurrence of CHF and that of MODSE were positively correlated with age (r = 0.696 - 0.987, P < 0.01). High risk population of MODSE induced by CHF were old patients with hypertension above 69 year old.</p><p><b>CONCLUSION</b>The age is valuable for early prediction of MODSE induced by CHF in old patients with hypertension. The distinctly boundary age for the incidence of MODSE induced by CHF in old patients with hypertension is 69.</p>

Study on noninvasive measurement of blood glucose based on optical rotation / 生物医学工程学杂志

With the development of economy, the incidence of diabetes is keeping on rising. It has been a larger chief offender endangering human health. Glucose monitoring in time, accurately and continuously can provide the basis for the adjustment of diet, exercise and drug treatment project, and can control disease at the level of satisfaction degree. Noninvasive measurement of glucose avoids blood collection with high frequency, alleviates pain caused by blood sampling, and prevents infection. It comes with hope for the diabetic. In this article, we compare the kinds of techniques, introduce the theory, the problems of polarization rotation, the solving methods and the advantages, thus providing references for the noninvasive measurement of glucose.

Application of comparison method in internal quality control of hematology analyzer by using fresh blood / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the comparison method on internal control of hematology analyzer by using fresh blood.</p><p><b>METHODS</b>The hematology analyzer with well function was selected as the reference analyzer, fresh blood samples from healthy subjects were measured by reference analyzer and the values were used to calibrate compared hematology analyzers. The acceptable limits of relative deviation of WBC,RBC, HGB,HCT, PLT were established by comparative experiments during three months. The results of fresh blood samples from patients with low/medium/high levels measured by compared analyzer were compared with those from reference analyzer, the relative deviation of WBC, RBC, HGB, HCT, PLT was calculated respectively. The internal quality control charts in laboratory information system were established, with date as x-axis, relative deviation as y-axis. The acceptable relative deviation limits were set to be +/-2 s, and to be used for laboratory quality control.</p><p><b>RESULT</b>The relative deviation of WBC, RBC, HGB, HCT, PLT with high, medium, low levels were(0.75+/-2.964)%, (1.19+/-2.488)%,(1.43+/-2.439)%; (-0.39+/-1.327)%, (-0.26+/-1.297)%, (-0.35+/-1.095)%û(-0.43+/-1.393)%, (-0.17+/-1.139)%, (0.24+/-1.166)%û(-.43+/-1.362)%, (-0.36+/-1.381)%, (-0.57+/-1.299)%û(-0.93+/-4.330)%,(0.04+/-4.118)%, (-0.41+/-4.149)%, respectively in 2006. As the second instrument, the compared analyzer was involved in College of American Pathologists Proficiency Testing with satisfactory results, the bias of WBC,RBC, HGB, HCT, PLT were within (-0.5 approximately 5.1)%, (-1.0 approximately 1.6)%, (-1.7 approximately 1.4)%, (-1.5 approximately 1.3)%, (-4.5 approximately 7.4)%, respectively.</p><p><b>CONCLUSION</b>The quality control on compared hematology analyzer can be effectively, conveniently and economically performed using this method.</p>

Construction and sequencing of full-length cDNA clone of swine vesicular disease virus strain HK'1/70 / 病毒学报

By RACE, 2 overlapping cDNA fragments (3'PCR and 5'PCR fragments) covering the full genome of swine vesicular disease virus strain HK'1/70 were amplified from total RNA extracted from experimentally infected suckling mice. These fragments were cloned into pGEM-T Easy vector, respectively. 5'PCR fragment was digested by enzymes of Aat II and BssH II, and the Aat II-BssH II-digested 5'PCR fragment was obtained and cloned into the recombinant pGEM-T Easy vector containing 3'PCR fragment,the recombinant plasmid encoding full-length cDNA of SVDV HK'I/70 strain was then obtained and sequenced. The results showed that the complete genome of HK'1/70 was 7401 nucleotides (nts) long (excluding the poly (A) tract) which encodes a single polyprotein of 2185 amino acids, a 5'u ntranslating region (UTR) of 743 nts, a 3'UTR of 102 nts and a poly (A) tail at least 74 adenines. T' promoter was added at the 5'e nd of the full-length cDNA and an additional Pspl406I restriction site was added at the 3'e nd of poly (A) tail. The nucleotide and amino acid sequences were compared and phylogenetic analysis was used to examine the evolutionary relationships. The results showed that HK'1 /70 belonged to the second antigenic group. SVD virus was antigenically closely related to Coxsackie B5 virus, and located on the branches of CB5 evolutionary tree. Successful construction of full-length cDNA clone of SVDV HK'1/70 strain lays foundation for rescuing SVDV effectively and enables further research of SVDV on molecular level.

Utilization of Drugs in Children with Ventricular Septal Defect:Analysis of 120 Cases / 中国药房

OBJECTIVE:To analyze the utilization of drugs in children with ventricular septal defect(VSD) undergoing operation.METHODS:The medical orders of 120 VSD children patients in our hospital in 2005 were analyzed retrospectively.RESULTS:The per capita drug cost was(3 067.20? 325.04) yuan,accounting for 9.52% of the total medical cost.Circulatory system drugs and antibiotics were the chief categories.Leading the first 3 places in terms of DDDs were cefuroxime,vecuronium bromide and furosemide,respectively.CONCUSION:The drug cost for VSD children patients was relatively low and reasonable.

Optimization of the Formula of Retinoid Self-Emulsifying Preparation / 中国药房

OBJECTIVE: To optimize the formula of retinoid self-emulsifying preparation. METHODS: The formula of retinoid self-emulsifying drug delivery system was optimized with the solubility of retinoid in different kinds/proportions of oil phases and surfactants as index through solubility test and drawing of ternary phase diagrams. RESULTS: The optimized formula ratio of retinoid self-emulsifying preparation was 0.01∶0.938∶0.402∶0.15 (retinoid / IPM/ Tween-85/ isopropanol, g∶g∶g∶mL). CONCLUSION: The retinoid self-emulsifying preparation prepared in accordance with the above-mentioned ratio is up to the related standard.

The Effect of Piper Hancei Extract on the Atherosclerosis of Rabbits / 中国药房

OBJECTIVE:To observe the effect of Pipers hancei extract on the atherosclerosis of rabbits fed with high lipid food and to discuss its mechanism of action.METHODS:The rabbits were divided into high lipid group,Pipers hancei groups (high,medium and low dose groups),which were fed with high lipid food for 3 weeks before administered with medicine or normal saline,12 weeks later,the blood lipid,pathological changes of aortic plaque and lesion of coronary artery,serum superoxide dismutase (SOD)activity and serum malondialdehyde (MDA)activity were determined,in which a control group was established.RESULTS:Compared with the high lipid group,the levels of serum total cholesterol (TC),triglycerides (TG)and low density lipoprotein (LDL)have been significantly decreased for the high dose Pipers hancei group(P

Prescription screening of dispersible compound melatonin tablets / 第三军医大学学报

Objective To prepare dispersible compound melatonin tablets which can be quickly effective and used conveniently Methods The effective factors in the ingredients of the dispersible compound melatonin tablets were observed by orthogonal experiment design and the prescription was screened by disintegrating time limit Results The dispersible tablets could disintegrate completely in 50 seconds and the suspension could pass through 710 nm sifter in the dispersing test The dissolution rate could reach 92 9% in 5 minutes Conclusion The dispersible compound melatonin tablets can be prepared conveniently and easily disintegrate and dissolve

Preparation and Quality Control of Xinyoukexing Tincture / 中国药房

OBJECTIVE:To investigate the preparation technic and method of quality control of Xinyoukexing tincture(XYKX) METHODS:To determine the content of Valaciclovir by spectrophotography,and podophyllotoxin by dual-wavelength spectrophotography RESULTS:In the range of 14 88～34 72?g/ml,the Valaciclovir concentration in XYKX was in direct proportion to the absorption at 310 4nm,C=44 5 905A+1 7 556,r=0 9 998 In the range of 17 50～50 12?g/ml,the podophyllotoxin concentration in XYKX was in direct proportion to the absorption at 292nm,C=97 7 981Ap292+1 3 614,r=0 9 999,AP292=As292-As325 CONCLUSION:The preparation technic of XYKX is simple and the method of quality control is feasible

Effect of Concentrations of Alcohol in Precipitation Method on Polysaccharide Contents in Xinmaishen Oral Liquid / 中国药房

OBJECTIVE:To study the effect of concentrations of alcohol in precipitation method on polysaccharide contents in order to optimize the extractive condition of Xinmaishen oral liquid METHODS:Taking the total polysaccharides in the decoction as target and dertermining the effective components by phenol-vitriol colorimetry,the effects of 4 alcohol concentrations(50%,60%,70%,80%) on polysaccharide contents were studied RESULTS:The results showed that the polysaccharide contents sharply decreased with the increase of alcohol concentration When the alcohol concentration reached 80%,the polysaccharide content lost nearly 70% CONCLUSION:Polysaccharide content should be used as one of exam indices in screening preparation technique of this oral liquid

Study on Quality Standard for Shendi Tangmaining Capsules / 中国药房

OBJECTIVE:To identify Salvia miltiorrhiza,Rehmannia,Chuangxion rhizome,rhubarb and to determine the content of tanshinone Ⅱ A,as an efficacious composition,in Shendi Tangmaining capsules.METHODS:TLC and SPE-UV were used.The SPE-UV method was performed with silica gel column(1.1cm).The mobile phase consisted of dichloromethane-me_thanol(8∶2).The detected wavelength was 280nm.RESULTS:In the SPE-UV method,the standard curve for tanshinone Ⅱ A was linear in the range of 1.6～8?g/ml(r=0.9 999).The average recovery was 96.13% with RSD=0.80%.CONCLUSION:The method is simple,rapid and reliable for quality control of the capsules.